PEG MGF vs MGF

[G21]

Here it is plain and simple for you. Without any complex peptide understanding required other than the fact that PEGylation increases the stability, and lifetime of the peptide.

MGF is responsible for increasing muscle stem cell count through a process called proliferation.



As you see the effect going left to right here is exponential. The more stem cells you have the more that can be stimulated and replicated. In order to singal this replication there has to be sufficient saturation of cell receptors. Because attachment to a receptor is by simple probability, the more peptide and the more time the more probable a cell will reach saturation and begin to replicate.

IF regular MGF has a shorter life span, it has a significantly smaller chance at saturating cells. It is not stable in the blood so its only real chance to bind is right at injection. If you calculate probability of binding via (time x concentration) and then PEG last appx 300 times longer, the probability of MGF having a significant effect is about 300 times at the same dosage. In other words you would need 300 times the regular MGF to bind to as many receptors as the PEGylated version. And even at that case the loss of peptide to the blood so fast would still limit the effects of MGF.

Now you must also consider the cumulative effects of having longer term MGF action. AS you saw in the image, the number of receptors will increase, this increase the probability further for the PEG version so now every time you undergo muscle stem cell replication within the 4-8 days the PEG version is active you increase the probability proportional to the increase in cell number.

So without knowing anything more than multiplication and the law of probability one can see that PEG MGF is significantly more effective.

bron: IBE


dit verklaard de relatief lagere doseringen die wekelijks worden gezet met de PEG versie..

ben benieuwd hoeveel van deze theorie daadwerkelijk in de praktijk tot uiting komt..
__________________

[Leviathan]

De kortwerkende versie werkt volgens mij ook prima ( in combinatie met igf-1 lr3 en AAS).

Ik wilde met name mn biceps/triceps verbeteren, en heb inmiddels een mooie piek op mn bicep en de totale armomvang is ongeveer 1cm toegenomen.

Ik krijg overal te horen dat ik droger ben geworden en toch voller.
Kan kloppen, mn BF is iets omlaag gegaan en ben ondertussen toch een kleine 4kg aangekomen.

Armen zijn vasculairder geworden.

Om echt te kunnen zien in wat voor mate de MGF werkt, moet je het denk ik n keer zonder igf gebruiken, ook al zorgt die combinatie voor de beste effecten naar het schijnt.

[IKKE]

Effe update..

3 fabrikanten zijn anti peg
1 is pro peg (ibe)

[loekie]

PEG is de langwerkende versie toch?

[klaas]

tsja ... ben benieuwd, zit dus maar 1 ding op en dat is gebruiken die troep

[G21]

Origineel gepost door klaas

zit dus maar 1 ding op en dat is gebruiken die troep

lol..idd

[G21]

Origineel gepost door IKKE

Effe update..

3 fabrikanten zijn anti peg
1 is pro peg (ibe)

Here is a key article about MGF PEGylation...I have only pasted the applicable information to our discussion here but the article has been cited.
I have a few comments that I am highlighting in green to further explain the statements from the article and I have highlighted key points from the article in red.......

The FASEB Journal express article 10.1096/fj.05-3786fje.
Published online September 6, 2005
A strong neuroprotective effect of the autonomous Cterminal
peptide of IGF-1 Ec (MGF) in brain ischemia
Joanna Dłużniewska,* Anna Sarnowska,† Małgorzata Beręsewicz,* Ian Johnson,‡Surjit K. S. Srai,§ Bala Ramesh,§ Geoffrey Goldspink,|| Dariusz C. Górecki,¶ and Barbara Zabłocka*

……………………
Peptide synthesis
The 24 amino acid peptide amide (NH2-YQPPSTNKNTKSQ(d)R(d)RKGSTFEEHK-NH2 corresponding to the shorter consensus mammalian sequence for the C-terminal MGF peptide was synthesized using the Fmoc protection strategy. The D-form of arginine was used for the synthesis instead of the naturally occurring L-form. The N terminus was modified by a PEG derivative (O’O-bis(2-aminopropyl)polyethylene glycol 1900) (Jeffamine) via a succinic acid bridge.
……………………
Comments: Goldspink is the scientist that actually discovered MGF and for purposes specific to this study he uses MGF but instead of the human sequence which contains 3 arginines, they use the shorter consensus mamalian sequence containing 2 arginines. They removed the arginine at the 23rd position. Human form MGF has a third arginine at the 23rd position. They are also using the D-form of arginine to keep them from cleaving and makes it more stable.

Based on these analyses and taking into account the high degree of homology, we have generated a consensus sequence for the mammalian 24aa C-terminal peptides of the IGF-1 splice variant IGF-1Eb/Ec or MGF (Fig. 1B). This consensus sequence was used to prepare a synthetic, 24 amino acid long MGF C-terminal peptide. Based on the human sequence, this peptide contained two rather than three arginines (Fig. 1B). To increase the stability of the MGF peptide, which was found to be rapidly degraded in the serum or tissue fluids (Yang and Goldspink, unpublished), the D-form of arginine was used for synthesis instead of the naturally occurring Lform. Moreover, the N terminus of the peptide was PEGylated.………………………….....
Comments: As you can see proof here that plain MGF is not stable and must be PEGylated to stabilize.....from the mouth of Goldspink himself.

Such a small and biologically active molecule is a good candidate for development into a therapeutic modality. Indeed, we have shown here that C-terminal MGF peptide can be modified into a more stable and potentially targetable moiety by specific chemical modifications. In contrast, the recombinant full-length IGF-1, although neuroprotective in vitro, was found ineffective when delivered by intracarotid injection. It remains to be elucidated whether this disparity was due to different biological activities or resulted from the increased stability of the modified C-terminal MGF molecule. The difference in permeability through the blood-brain (BBB) barrier could also be a factor, but it has to be stressed that the PEGylated C-terminal MGF and recombinant IGF-1 have similar molecular size.
We demonstrate here the efficacy of the modified C-terminal MGF peptide administered as a single bolus injection intra-arterially. This suggests that the stabilized, pegylated peptide molecule was able to cross the BBB and penetrate into the neurones…………………..
Comments: As you can see clearly in red that PEGylation made the MGF peptide more stable and more targetable for development of a therapeutic modality for the pharmaceutical world.

*Molecular Biology Unit and †Department of NeuroRepair, Medical Research Centre, Polish
Academy of Sciences, Warsaw, Poland; ‡Department of Anatomy, §Department of Biochemistry
and Molecular Biology, and ||Molecular Tissue Repair Unit, Department of Surgery, Royal Free
and University College Medical School, London, England; ¶Institute of Biomedical and
Biomolecular Sciences, School of Pharmacy and Biomedical Sciences, University of
Portsmouth, England
Geoffrey Goldspink, Dariusz C. Górecki, and Barbara Zabłocka are senior coauthors.
Corresponding authors: Dariusz C. Górecki, Institute of Biomedical and Biomolecular Sciences,
St Michael’s Building, White Swan Road, Portsmouth, PO1 2DT, UK. E-mail:
darek.gorecki@port.ac.uk; or Barbara Zabłocka, Medical Research Centre, Polish Academy of
Sciences ul. Pawi

een Post van IBE zelf op anabolicminds..waarin ze toegeven dat de regular MGF useless is...

http://anabolicminds.com/forum/igf-1...-mgf-will.html

[IKKE]

Alleen alle andere fabrikanten zeggen dat peg juist mgf kapot maakt..en niet nodig..
Ook paar experts zoals anthony robberts..

[G21]

Origineel gepost door IKKE

Alleen alle andere fabrikanten zeggen dat peg juist mgf kapot maakt..en niet nodig..
Ook paar experts zoals anthony robberts..

...aha..tja het blijft iets wat zichzelf nog moet bewijzen..niet?!

anthony roberts vind ik trouwens een boef..op T-nation heb ik dikwijls artikelen gelezen waarin hij specifieke merken afkraakt..en andere weer helemaal ophemelt..achteraf blijkt dan dat hij onder contract staat voor bepaalde merken etc. niet echt geheel un-biased..zoals ze dat zeggen..

[IKKE]

Yep hij is ook beetje gerelateerd met de huidige sponsor van steroid.com forums.

[koen85]

Hmm, hij schrijft toch ook bijna alle artikelen voor mesomorphosis?

[G21]

Origineel gepost door koen85

Hmm, hij schrijft toch ook bijna alle artikelen voor mesomorphosis?

yep!